Status of endothelial progenitor cell in murine model of Kawasaki disease

• Published in: Zhong hua yi xue za zhi Vol. 92; no. 22; p. 1560
• Main Authors: Liu, Jun-feng, DU, Zhong-dong, Chen, Zhi, Li, Shen-tao, Lu, Dun-xiang, Li, Li
• Format: Journal Article
• Language: Chinese
• Published: China 12.06.2012
• Subjects: Animals; Disease Models, Animal; Down-Regulation; Endothelial Cells - cytology; Male; Mice; Mice, Inbred C57BL; Mucocutaneous Lymph Node Syndrome - metabolism; Mucocutaneous Lymph Node Syndrome - pathology; Stem Cells - cytology
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2012.22.016

To assess whether the occurrence of coronary artery lesion was correlated with the changes of endothelial progenitor cell (EPC) number and function in murine model of Kawasaki disease (KD). Lactobacillus casei cell wall extract (LCWE) was prepared and then C57BL/6 mice received a single intraperitoneal injection of LCWE for inducing KD. Twenty-four mice were categorized randomly into 3 groups: KD model group at Day 14 post-injection, KD model group at Day 56 post-injection and control group with an intraperitoneal injection of phosphate buffered solution (n = 8 each). The number of circulating EPC was defined as CD34(+)Flk-1(+)CD45(-) from mice. Meanwhile, bone marrow mononuclear cells were cultured in vitro to expand EPC for functional analysis. After 7 days of culturing, EPC were inoculated onto culture plate and thiazolyl blue assay was used to measure the absorbance value by enzyme labeling instrument to evaluate the proliferation. The adhesion of EPC was performed by replating cells on fibronectin coated