Association study of CNR1, GAD1 and BDNF polymorphisms with male heroin dependence in the Dai population in Yunnan

• Published in: Yíchuán Vol. 36; no. 9; p. 888
• Main Authors: Yang, Yaxian, Chen, Yanxiang, Wu, Jianquan, Xing, Yuming, Zeng, Faming, Huang, Youhao, Cheng, Bao Wen
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2014
• Subjects: Adult; Alleles; Base Sequence; Brain-Derived Neurotrophic Factor - genetics; Brain-Derived Neurotrophic Factor - metabolism; Case-Control Studies; China - ethnology; Gene Frequency; Glutamate Decarboxylase - genetics; Glutamate Decarboxylase - metabolism; Haplotypes; Heroin Dependence - ethnology; Heroin Dependence - genetics; Humans; Male; Middle Aged; Molecular Sequence Data; Polymorphism, Single Nucleotide; Receptor, Cannabinoid, CB1 - genetics; Receptor, Cannabinoid, CB1 - metabolism; Young Adult
• ISSN: 0253-9772
• DOI: 10.3724/SP.J.1005.2014.0888

In order to analyze the association of CNR1(Cannabinoid receptor 1), GAD1(Glutamate decarboxylase 1), and BDNF(Brain-derived neurotrophic factor) polymorphisms with male heroin dependence in the Dai population in Yunnan Province, an eight-SNP co-amplification protocol was established to genotype on the SNaPshot platform. A case-control study was performed with 8 SNPs from CNR1, GAD1, and BDNF genes in 165 heroin-dependent males and 170 healthy males of the Dai population. Statistical analyses were conducted with SPSS17.0, Haploview4.2, PHASE2.1, and MDR software. We found that: (1) the genotype frequency of rs13306221 was significant in the case group (P<0.025); (2) the A allelic frequency of rs6265 was significantly higher in the case group; (3) the haplotypes of T-A-C, C-C-C, C-C-T, and T-C-C based on rs1978340-rs3791878-rs11542313 and haplotype A-G based on rs6265-rs13306221 were significant (P<0.05); (4) the haplotype frequencies of T-A-C, C-C-T, and A-G were significantly higher in the case group. These