The relationship between hypermethylation of Syk gene promoter and medulloblastoma cell invasion and metastasis

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 32; no. 2; p. 132
• Main Authors: Ji, Hai-Long, Shi, Peng-Fei, Zhou, Jie, Mao, Tian-Ming, Luo, Yong-Kang, Chen, Xi, Zhou, Kai-Yu
• Format: Journal Article
• Language: Chinese
• Published: China 08.02.2016
• Subjects: Azacitidine - pharmacology; Cell Line, Tumor; Cell Movement; Cerebellar Neoplasms - metabolism; DNA Methylation; Gene Expression Regulation, Neoplastic; Humans; Medulloblastoma - metabolism; Neoplasm Invasiveness; Promoter Regions, Genetic; Syk Kinase - genetics; 5-aza-CdR; medulloblastoma; Syk gene; invasion and metastasis; methylation
• ISSN: 1000-6834
• DOI: 10.13459/j.cnki.cjap.2016.02.010

To investigate the effect of demethylation of Syk gene promoter by the methylation transferase inhibitor 5-aza-CdR on the invasion and metastasis of medulloblastoma cell line Daoy. Medulloblastoma cell line Daoy was treated with 5-aza-CdR Methylation-specific PCR, real time-PCR and Western blot were used to detect Syk gene promoter methylation status, Syk mRNA and protein expression respectively. Transwell was employed to study the invasion and metastasis of medulloblastoma cell line Daoyby counting the cells that had invaded through Matrigel and migrated to the undersurface of the membrane before and after treatment of 5-aza-CdR. In comparison to control group, Syk gene promoter of 5-aza-CdR-treated groups was demethylated and expression of Syk mRNA and protein was significantly up-regulated by 3.40±0.24 folds ( <0.01) and 3.23±0.19 folds ( <0.01) respectively. The invasiveness and metastasis of medulloblastoma cell line Daoy was decreased( <0.05). Hypermethylation of Syk gene promoter is responsible for the