Effect of electroacupuncture pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP) signal and NF-κB p65 protein expression in rats with acute myocardial ischemia

• Published in: Zhēncì yánjiū Vol. 46; no. 1; p. 58
• Main Authors: Wu, Jia-Hong, Sun, Ke, Lu, Sheng-Feng, Bai, Hua, Xu, Sen-Lei, Xia, Xue-Feng, Liu, Yu-Chen, Gu, Yi-Huang, Zhang, Hong-Ru
• Format: Journal Article
• Language: Chinese
• Published: China 25.01.2021
• Subjects: Acupuncture Points; Animals; Calcitonin; Calcitonin Gene-Related Peptide - genetics; Electroacupuncture; Male; Myocardial Ischemia - genetics; Myocardial Ischemia - therapy; NF-kappa B - genetics; Rats; Rats, Sprague-Dawley; TRPV Cation Channels - genetics; Acute myocardial ischemia;Electroacupuncture pretreatment;Transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP); Nuclear factor-κB p65 (NF-κB p65)
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.200905

To observe the effect of electroacupuncture(EA)pretreatment on transient receptor potential vanilloid 1(TRPV1)/calcitonin gene-related peptide(CGRP)signal and nuclear factor-κB p65 (NF-κB p65) protein expression in myocardial tissue of acute myocardial ischemic injury (AMI) rats, and to investigate the possible mechanism of electroacupuncture pretreatment against AMI. A total of 60 adult male SD rats were randomly divided into blank control, sham operation, model and EA pretreatment groups, 15 rats in each group. The acute myocardial ischemia model was established by ligating the left anterior descending (LAD)branch of the coronary artery in the model group and EA pretreatment group, while threading but no ligating at left anterior descending branch of the coronary artery was applied in the sham operation group. In the EA pretreatment group, bilateral "Neiguan"(PC6) acupoints were selected, with intensity of 2 mA and frequency of 2 Hz/100 Hz, for 20 min, once daily for 7 days before modeling. Electrocardiogra