Effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 35; no. 1; p. 60
• Main Authors: Xie, Ke-Jian, Huang, Ling, Qu, Xiao-Hu, Li, Xue, Wang, Shao-Jie, Xiao, Min
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2019
• Subjects: Animals; Diabetes Mellitus, Experimental; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - drug therapy; Inflammation; Insulin; Insulin Receptor Substrate Proteins - drug effects; Male; Mice; Pancreas; Piperidines - pharmacology; Random Allocation; Receptor, Insulin - drug effects; Spleen - drug effects
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5749.2019.015

To observe the effects of AdipoRon orally on the functions of spleen and pancreas in type 2 diabetic mice, in order to present data for clinical application. Forty C57/BL6 male mice were randomly divided into 2 groups: normal control group (n=10) and model group (n=30), the former group was fed normally, while the later group was fed with high fat and sugar for 4 weeks.After that, type 2 diabetes model was established in DM group induced by intraperitoneal injection of streptozotocin (STZ, 40 mg/kg).As type 2 diabetes model established successfully, the model mice were randomly divided into three groups (n=10): diabetes mellitus (DM) group, high dose of AdipoRon group (DM + H) and low dose of adiponRon group (DM + L).All the four groups were treated with saline, saline, AdipoRon at the doses of 20 mg/kg and 50 mg/kg by gavages respectively, once a day for 10 days.And then put them to death for collecting blood, pancreas and spleen.Pathological changes of pancreas were observed with a light microscope after HE