Effect of Shenfu injection on expression of lipopolysaccharide --induced microRNA-146a in alveolar macrophages

• Published in: Zhongguo wei zhong bing ji jiu yi xue Vol. 24; no. 3; p. 166
• Main Authors: Zeng, Zhen-guo, Gong, Hong-han, Li, Yong, Nie, Zhen-yun, Jie, Ke-min, Zhan, Yi-an, Nie, Cheng, Liu, Fen, Ding, Cheng-zhi, Shao, Qiang, Qing, Cheng, Zhu, Bai-lu, Qian, Ke-jian
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2012
• Subjects: Animals; Cell Line; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - pharmacology; Lipopolysaccharides; Macrophages, Alveolar - cytology; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - metabolism; MicroRNAs - metabolism; Rats; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1003-0603
• DOI: 10.3760/cma.j.issn.1003-0603.2012.03.013

To study the effects of Shenfu injection (SF) on the expression of lipopolysaccharide(LPS)-induced microRNA-146a (miR-146a) in rat alveolar macrophages (AMs), and to extrapolate its potential anti-inflammatory mechanisms. In vitro cultured rat AMs (NR8383 cells) were randomly divided into control group, LPS stimulation group, and SF stimulation group. The LPS stimulation group was challenged with a final concentration of 1 mg/L LPS, and to the control group an equal volume of phosphate buffer solution (PBS) was added instead. For SF treated group, SF in different concentrations (1 ml/L or 10 ml/L) was used during incubation of AMs for half an hour, and then LPS was added (1 mg/L final concentration). After 6 hours, the cells and were collected. MiRNA-146a expression [reverse transcription-polymerase chain reaction (RT-PCR)] in cells and tumor necrosis factor-α (TNF-α ) content [enzyme-linked immunosorbent assay (ELISA)] in culture supernatant were determined for each group. Both the expression of miR-146a and