Chinese herbs for shen invigorating and blood activating activated MMP-9 signaling pathway to mobilize rats' bone marrow EPCs: a molecular mechanism research

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 33; no. 6; p. 795
• Main Authors: Dai, Guo-Hu, Zhang, Tong, Wu, Bo, Jiao, Hua-Chen, Liu, Yuan-Feng, Song, Xian-Bo
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2013
• Subjects: Animals; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Drugs, Chinese Herbal - pharmacology; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Male; Matrix Metalloproteinase 9 - metabolism; Myocardial Infarction - metabolism; Rats; Rats, Sprague-Dawley; Signal Transduction; Stem Cells - drug effects; Stem Cells - metabolism
• ISSN: 1003-5370

To explore the effect of Chinese herbs for Shen invigorating and blood activating (CHSIBA) on the number of endothelial progenitor cells (EPCs) in the bone marrow and the peripheral blood and the signaling pathway of bone marrow matrix metalloproteinase 9 (MMP-9) of the myocardial infarction (MI) model rats. The MI rat model was established by ligation. Thirty successfully modeled rats were randomly divided into the high dose CHSIBA group, the low dose CHSIBA group, and the model group, 10 in each group. Besides, another 10 normal rats were recruited as the blank group. Rats in the high dose CHSIBA group and the low dose CHSIBA group were administered with CHSIBA at 3 g/kg and 1.5 g/kg body weight by gastrogavage (by adding them in 4 mL physiological saline), once daily. Rats in the model group and the blank group were administered with 4 mL physiological saline once daily. The EPCs were collected from the bone marrow and the peripheral blood 4 weeks later. Seven days later the CD34/CD133 phenotype was identi