Effects of tramadol hydrochloride preconditioning on the activation of nuclear factor-ΚB induced by norepinephrine in cultured neonatal rat cardiomyocytes

• Published in: Zhonghua wei zhong bing ji jiu yi xue Vol. 25; no. 11; p. 646
• Main Authors: Lü, Zhi-gan, Guo, Zheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2013
• Subjects: Animals; Animals, Newborn; Cells, Cultured; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; NF-kappa B - metabolism; Norepinephrine - pharmacology; Rats; Rats, Sprague-Dawley; Tramadol - pharmacology
• ISSN: 2095-4352
• DOI: 10.3760/cma.j.issn.2095-4352.2013.11.003

To investigate the effects of tramadol hydrochloride preconditioning on activation of nuclear factor-ΚB (NF-ΚB) induced by norepinephrine (NE) in cultured neonatal rat cardiomyocytes. 1 to 3-day old Sprague-Dawley (SD) rats were raised, and their cardiomyocytes were cultured for 4 to 5 days. Sixteen wells of cultured cardiomyocytes were randomly divided into four groups (n=4): blank control group, NE1 group in which the cells were incubated with 1×10(-6) mmol/L of NE, NE2 group in which cells were incubated with 1×10(-7) mmol/L of NE, and NE3 group in which cells were incubated with 1×10(-8) mmol/L of NE. Another 12 wells of cultured cardiomyocytes were randomly divided into three groups (n=4): blank control group, NE3 group in which cells were incubated with 1×10(-8) mmol/L of NE, tramadol plus NE group (T + NE group) in which cells were incubated with 1×10(-5) mmol/L of tramadol hydrochloride plus 1×10(-8) mmol/L of NE. Tramadol hydrochloride was added 30 minutes before the use of NE in T + NE group. After