Effects of local transplantation of autologous adipose-derived mesenchymal stem cells on the formation of hyperplastic scar on rabbit ears

• Published in: Zhonghua shao shang za zhi Vol. 32; no. 10; p. 582
• Main Authors: Chen, L, Wang, D L, Wei, Z R, Wang, B, Qi, J P, Sun, G F
• Format: Journal Article
• Language: Chinese
• Published: China 20.10.2016
• Subjects: Animals; Cicatrix, Hypertrophic; Collagen; Collagen Type I; Collagen Type III; Decorin; Dermis; Ear; Mesenchymal Stromal Cells; Rabbits; Transforming Growth Factor beta1; Up-Regulation; Wound Healing
• ISSN: 1009-2587
• DOI: 10.3760/cma.j.issn.1009-2587.2016.10.003

To investigate the effects of local transplantation of autologous adipose-derived mesenchymal stem cells (ADSCs) on the formation of hyperplastic scar on rabbit ears. ADSCs were isolated from inguinal fat of six New Zealand rabbits and then sub-cultured. ADSCs of the third passage of each rabbit were used in the following experiments. Six full-thickness skin defect wounds with diameter of 6 mm on the ventral surface of every rabbit ear were made. Wound healing and local-tissue proliferation were observed, and complete epithelization time of wounds and formation time of hyperplastic scar were recorded. The wounds on left ears were selected as group ADSCs, and the wounds on right ears were selected as control group, with 36 wounds in each group. After the complete epithelization of wounds (post injury day 25), 0.2 mL bromodeoxyuridine (BrdU) labeled autologous ADSCs with the concentration of 5×10 per milliliter were injected into each wound of the rabbit of group ADSCs, while the same amount of phosphate buffer