Effect of Electroacupuncture and Moxibustion Pretreatment on Expression of Autophagy Related Proteins LC 3 and Beclin 1 in Rats with Myocardial Ischemia-reperfusion Injury

• Published in: Zhēncì yánjiū Vol. 43; no. 1; p. 1
• Main Authors: Tan, Cheng-Fu, Wang, Chao, Du, Lin, Liu, Wei-Wei, Song, Jin, Feng, Guo, Yan, Jie, Yang, Jing-Jing, Tang, Ya-Ni, Chen, Mei-Lin, Li, Jiao-Lan
• Format: Journal Article
• Language: Chinese
• Published: China 25.01.2018
• Subjects: Animals; Beclin-1; Electroacupuncture; Microtubule-Associated Proteins; Moxibustion; Myocardial Reperfusion Injury; Rats; Rats, Sprague-Dawley; Electroacupuncture pretreatment; Myocardial autophagy proteins; Ischemic preconditioning; Myocardial ischemia-reperfusion; Moxibustion pretreatment
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.170181

To observe the effect of electroacupuncture (EA) and moxibustion (Moxi) pretreatment on myocardial pathological and structural changes and expression of autophagy related protein LC 3 Ⅰ/Ⅱ and Beclin 1 in rats with myocardial ischemia-reperfusion injury (MI/RI), so as to explore their mechanisms underlying improving MI/RI. Forty SD rats were randomly divided into sham operation, model, ischemic preconditioning (IP), EA and Moxi groups ( ＝8 in each group). EA (10 Hz/50 Hz，1 mA) or Moxi (ignited moxa stick) was respectively applied to bilateral "Neiguan"(PC 6) for 20 min, once daily for 7 days. The MI/RI model was established by occlusion of the anterior descending branch of the left coronary artery for 40 min, followed by reperfusion for 60 min. The left ventricular (LV) tissue samples were collected and analyzed for pathological (H.E. staining) and ultrastructural changes, for myocardial apoptosis (apoptotic index＝ number of apoptotic cells/total number of cardiomyocytes×100%) with terminal deoxynucleotidyl tr