Effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells

To investigate the effect of overexpressing rat aldo-keto reductases (AKR7A1) in V79-4 cells on crotonaldehyde-induced mutagenicity. The expression level and enzyme activity of AKR7A1 protein expressed in V79-4 cells were measured by Western blotting and AKR enzyme assay, respectively. HGPRT assay w...

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Bibliographic Details
Published inXi bao yu fen zi mian yi xue za zhi Vol. 28; no. 10; p. 1013
Main Authors Li, Dan, Zhang, Qi-shan, Chu, Yang
Format Journal Article
LanguageChinese
Published China 01.10.2012
Subjects
Aldehyde Reductase - genetics
Aldehyde Reductase - metabolism
Aldehydes - pharmacology
Animals
Cell Line
Gene Expression
Hypoxanthine Phosphoribosyltransferase - genetics
Mutagenicity Tests
Mutagens - pharmacology
Mutation - drug effects
Mutation - genetics
Rats
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Summary:To investigate the effect of overexpressing rat aldo-keto reductases (AKR7A1) in V79-4 cells on crotonaldehyde-induced mutagenicity. The expression level and enzyme activity of AKR7A1 protein expressed in V79-4 cells were measured by Western blotting and AKR enzyme assay, respectively. HGPRT assay was applied to evaluate the effect of AKR7A1 overexpression on crotonaldehyde-induced mutagenicity in V79-4 cells. Western blotting confirmed a high expression level of AKR7A1 protein in V79-4 cells. AKR enzyme assay showed that the overexpressed AKR7A1 protein was functionally active. HGPRT assay demonstrated that cells overexpressing AKR7A1 exhibited a significant increase in resistance to crotonaldehyde-induced mutagenicity compared to control cells. Overexpression of AKR7A1 can protect V79-4 cells against crotonaldehyde-induced mutagenicity.
ISSN:1007-8738