Experimental study of hyaluronic acid on the biomechanical compliance of porcine acellular dermal transplantation

• Published in: Zhong hua yi xue za zhi Vol. 92; no. 20; p. 1428
• Main Authors: Zhao, Jing-yu, Chai, Jia-ke, Song, Hui-feng, Xu, Ming-huo
• Format: Journal Article
• Language: Chinese
• Published: China 29.05.2012
• Subjects: Acellular Dermis; Animals; Collagen Type I - metabolism; Collagen Type III - metabolism; Compliance; Hyaluronic Acid - pharmacology; Rabbits; Skin - drug effects; Skin Transplantation; Swine; Transplantation, Autologous; Transplantation, Heterologous
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2012.20.017

To explore the effect on biomechanical compliance after an addition of hyaluronic acid (HA) in transplantation of porcine acellular dermal matrix (PADM) with skin autograft. Ten Japanese white rabbits were used as experimental animal, the dorsa of rabbits was symmetrically divided into four area, and then grouping was randomized. Full-thickness skin defects were created in Group A to Group C, and Group D was blank group with normal skin. The following procedures were performed: Group A: implanted with HA+PADM+ thin skin autografts, GroupB: implanted with PADM+ thin skin autografts and Group C: skin autografts, except for Group D. The wound tissue specimens were harvested at 70 days postoperatively, and they were recorded contracture rates of the grafts and the biomechanical compliance by Instron biomechanics tensiometer. The expression of collagens I and III in dermal fibroblasts of each group was determined by Western blot. On postoperation day 70, the grafts contracture rates in Group A to Group C were 10.2