Lower frequency electroacupuncture is better in promoting recovery of limb locomotion in rats with sciatic nerve injury by reducing local inflammatory reaction

• Published in: Zhēncì yánjiū Vol. 44; no. 8; p. 571
• Main Authors: He, Qing-Xuan, Pan, Si-Teng, Chen, Yi-Ran, Ma, Tie-Ming
• Format: Journal Article
• Language: Chinese
• Published: China 25.08.2019
• Subjects: Animals; Electroacupuncture; Female; Locomotion; Male; Rats; Rats, Sprague-Dawley; Sciatic Nerve; Sciatic Neuropathy; Huantiao (GB30); Electroacupuncture; Limb locomotion; Sciatic nerve Injury; Inflammatory factor; Nuclear factor κB
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.180843

To observe the effect of different frequencies (2 Hz, 100 Hz) of electroacupuncture (EA) on limb locomotion and the expression of inflammatory factors IL-1β, IL-6, TNF-α in sciatic nerve, and nuclear factor kappa B (NF-κB) in lumber(L)4-L5of spinal cord in rats with sciatic nerve injury (SNI), so as to reveal its mechanisms underlying improvement of SNI. A total of 48 SD rats (half male and half female) were equally divided into blank control, model, low frequency (2 Hz) EA and high frequency (100 Hz) EA groups. The SNI model was established by clamping the spinal nerve. EA intervention (2 Hz, 100 Hz, 1 mA), starting on the 8 day after modeling, was applied to "Huantiao" (GB30) on the injured side for 15 min, once daily for 14 consecutive days. The sciatic function index (SFI) was calculated to assess the injured hindlimb recovery with reference to BAIN's and colleagues' methods. Histopathological changes of the sciatic nerve were displayed by H.E. staining. The expressions of IL-1β, IL-6 and TNF-α in the sci