Gender-dependent Expression of ERβ in AEC Ⅱ of Fetal Mice Exposed to Arsenic and Estrogen Receptor Antagonist

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 49; no. 3; p. 364
• Main Authors: Yang, Meng-Ping, Che, Wang-Jun, Cheng, Ya-Ling, Xiong, Lin-Sen, Zhang, Hao
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2018
• Subjects: Alveolar Epithelial Cells - drug effects; Alveolar Epithelial Cells - metabolism; Animals; Apoptosis; Arsenic - toxicity; Estrogen Receptor alpha; Estrogen Receptor Antagonists - toxicity; Estrogen Receptor beta - metabolism; Female; Male; Mice; Mice, Inbred ICR; Pregnancy; Sex Factors; Estrogen receptor β; Estrogen receptor antagonist; Fetal mice; Sodium arsenite; AEC
• ISSN: 1672-173X

To determine the effects of arsenic and estrogen receptor antagonist (ICI182, 780) on the expression of estrogen receptor beta (ERβ) in alveolar Ⅱ epithelial cells (AECⅡ) of female and male mice. Nineteen or twenty day fetus mice were obtained through caesarean section of ICR mice. Purified AECⅡ cells were separated from the female and male fetus,respectively,and confirmed using immunofluorescence staining. The cells were exposed to sodium arsenite (NaAsO ) at a low,medium,or high dosage determined by MTT and cultured for 24 h. The NaAsO (5 μmol/L) exposed cells were compared with those treated (for 24 h) with dimethyl sulfoxide (DMSO) or ICI182, 780 (1×10 mol/L). Apoptosis rates of the cells were measured by flow cytometry. Real-time fluorescence quantitative PCR method and Western blot technique were used to detect the expression of mRNA and protein in AECⅡ. Purity of AECⅡ cells reached (87.0±2.5)%. NaAsO exposure was set at a concentration of 0.5 (low),1.25 (medium),and 5 (high) μmol/L. The cells exposed t