Observing the effect of high glucose on proliferation of bone marrow stromal stem cells through Wnt/Β-catenin pathway

• Published in: Zhōngguó yīxué kēxuéyuàn xuébào Vol. 36; no. 4; p. 389
• Main Authors: Zhang, Bo, Liu, Na, Gu, Bin, Wu, Hao, Gao, Yu-xuan, Wang, Dong-sheng, Liu, Hong-chen
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2014
• Subjects: Animals; beta Catenin - metabolism; Bone Marrow Cells - cytology; Cell Proliferation - drug effects; Cyclin D1 - metabolism; Glucose - pharmacology; Lymphoid Enhancer-Binding Factor 1 - metabolism; Male; Mandible - cytology; Mesenchymal Stromal Cells - cytology; Rats; Rats, Wistar; Wnt Signaling Pathway
• ISSN: 1000-503X
• DOI: 10.3881/j.issn.1000-503X.2014.04.008

To explore the effect of high glucose on proliferation of bone marrow stromal stem cells through Wnt/Β-catenin pathway. Bone marrow stormal cells were obtained from the mandible of Wistar rats and stimulated with different concentrations of glucose (5.5 and 16.5 mmol/L). Cell proliferation was evaluated with methyl thiazolyl tetrazolium assay (1, 3, 5, and 7 d)and cell cycle analysis by flow cytometry (5 d). Β-catenin and cyclin D1 protein levels were determined by Western blot. The mRNA expression of lymphoid enhancer binding factor-1 (LEF-1) and cyclin D1 were tested by real-time polymerase chain reaction. The results of methyl thiazolyl tetrazolium assay indicated that the optical density values of two different concentrations of the glucose had no statistical difference on day 1 (P=0.700). On days 3, 5, and 7, the optical density values of the 16.5 mmol/L group were significantly lower than those in the 5.5 mmol/L group (P=0.006, P=0.002, and P=0.003). Cell cycle analysis indicated that high glucose conce