Effect of interleukin-17 on neutrophil apoptosis in patients with tuberculosis

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 30; no. 8; p. 833
• Main Authors: Jiang, Lina, Yao, Chunyan, Jin, Qili, Li, Baiqing
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2014
• Subjects: Adult; Apoptosis - drug effects; Butadienes - pharmacology; Cells, Cultured; Dose-Response Relationship, Drug; Female; Flow Cytometry; Humans; Interleukin-17 - pharmacology; Male; Middle Aged; Mitogen-Activated Protein Kinase Kinases - antagonists & inhibitors; Mitogen-Activated Protein Kinase Kinases - metabolism; Neutrophils - drug effects; Neutrophils - pathology; Nitriles - pharmacology; Time Factors; Tuberculosis - blood; Young Adult
• ISSN: 1007-8738

To investigate the effect of interleukin (IL)-17 on the apoptosis of neutrophils (PMNs) from peripheral blood of patients with pulmonary tuberculosis (TB) and explore the possible involved signaling pathway. The fresh isolated PMNs from peripheral blood of TB patients and healthy adults were cultured for 0-24 hours, and then stained with annexin V. The flow cytometry was used to measure cell apoptosis of PMNs. The fresh isolated PMNs were cultured with different concentrations of IL-17 for 24 hours, with or without pretreatment of MAPK inhibitor U0126 for 30 minutes, the apoptosis of PMNs was detected by flow cytometry. The apoptosis of PMNs in both TB patients and healthy adults increased with the culture time going on (P<0.05). The spontaneous apoptotic (annexin V⁺) rates of PMNs at 0, 6, 12 and 24 hours of culture time in TB patients were (4.49 ± 1.39)%, (21.89 ± 2.90)%, (39.96 ± 4.15)%, and (68.35 ± 7 .01)%, respectively, which were significantly higher than the corresponding ones in normal healthy groups