Quantitative and comparative proteomics analysis in clear cell renal cell carcinoma and adjacent noncancerous tissues by 2-D DIGE

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 37; no. 11; pp. 1517 - 1522
• Main Authors: Chen, Zhuang-Fei, Xiao, Yao-Jun, Huang, Ze-Hai, Chen, Tong, Zhao, Shan-Chao, Jiang, Yao-Dong, Wu, Peng, Zheng, Shao-Bin
• Format: Journal Article
• Language: Chinese
• Published: China 20.11.2017
• Subjects: Biomarkers, Tumor - metabolism; Carcinoma, Renal Cell - metabolism; Carrier Proteins; Electrophoresis, Gel, Two-Dimensional; Extracellular Matrix Proteins; Glycoproteins; Humans; Kidney Neoplasms - metabolism; Microfilament Proteins; Nuclear Proteins; Peroxiredoxins; Proteome - metabolism; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• ISSN: 1673-4254

To identify specific protein markers for renal cell carcinoma detection and diagnosis, as well as develop new potential therapeutic targets of the disease. We used two-dimensional difference in-gel electrophoresis (2-D DIGE) technique conjunction with mass spectrometry (MS) for the identification of significant differentially expressed proteins between 15cases of paired clear cell renal cell carcinoma (ccRCC) and adjacent normal renal tissues. The protein spots were considered as differentially expressed if a 1.5-fold altered expression level was observed (Student's t test, P value<0.05). Of the 27 differentially expressed protein spots, 26 proteins were successfully identified. 11 proteins up-regulated in renal cell carcinoma,15 proteins down-regulated. Among them Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACDSB), Aldose 1-epimerase (GALM), Peroxiredoxin-4 (PRDX4), Macrophage-capping protein (CAPG), Beta-defensin 107 (D107A), Microfibril-associated glycoprotein 4 (MFAP4) were first