Determination of serum carbamazepine concentration and metabonomic analysis in rats

To study the effects of carbamazepine on serum metabolic profiles in rats using nuclear magnetic resonance (NMR) spectroscopy. Twenty-four healthy male Wistar rats were randomized into 4 groups (n=6) for daily intragastric administration of high-, medium- or low-dose carbamazepine or distilled water...

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Published inNan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 34; no. 7; p. 1025
Main Authors Cai, Zhuo, Mo, Li-Qian, Guan, Shan-Yue, Liu, Chu-Yang, Liu, Yun, Guo, Dan
Format Journal Article
LanguageChinese
Published China 01.06.2014
Subjects
Animals
Carbamazepine - blood
Kidney - pathology
Liver - pathology
Magnetic Resonance Spectroscopy
Male
Metabolomics
Principal Component Analysis
Rats
Rats, Wistar
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Summary:To study the effects of carbamazepine on serum metabolic profiles in rats using nuclear magnetic resonance (NMR) spectroscopy. Twenty-four healthy male Wistar rats were randomized into 4 groups (n=6) for daily intragastric administration of high-, medium- or low-dose carbamazepine or distilled water (control) for 7 days. Blood samples were collected from the abdominal aortic under anesthesia after the treatment to determine serum carbamazepine concentration using high-performance liquid chromatography. ¹H nuclear magnetic resonance (¹H NMR) spectra were acquired for pattern recognition analysis. Histopathological changes of the renal and liver tissues of the rats were also examined. Steady-state blood concentration of carbamazepine in high-, medium- and low-dose groups were 14.64 ± 1.41, 8.54 ± 1.19, and 4.56 ± 0.64 µg/ml, respectively. Slight liver swelling was found in high-dose group, but none of the groups showed renal pathologies. Compared with the control group, the high-dose carbamazepine group showed
ISSN:1673-4254