Intervention of Qi-activating and Spleen-strengthening Herbs on Ca2+/CaMK II Signaling Pathways Key Factors in Skeletal Muscle Tissue of Rats with Spleen-qi Deficiency

• Published in: Zhong yao cai Vol. 38; no. 3; p. 562
• Main Authors: Duan, Yong-qiang, Cheng, Ying-xia, Liang, Yu-jie, Cheng, Wei-dong, Du, Juan, Yang, Xiao-yi, Wang, Yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2015
• Subjects: Animals; Calcium Signaling - drug effects; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Drugs, Chinese Herbal - pharmacology; Fabaceae - chemistry; Intestines; Muscle, Skeletal - drug effects; Muscle, Skeletal - enzymology; Plant Extracts - pharmacology; Plants, Medicinal - chemistry; Qi; Rats; Spleen
• ISSN: 1001-4454

To observe changes of [Ca2+]i concentration and CaM, CaMK II and p-CaMK II of Ca2+/CaMK II signaling pathways in skeletal muscle tissue of rats with spleen-qi deficiency and intervention of Sijunzi decoction and extract of Hedysarum polybotrys. Rats were randomized into four groups: normal control group, spleen-qi deficient model group, extract from Hedysarum polybotrys group and Sijunzi decoction group, ten rats in each group. After the spleen-qi deficient models were built by comprehensive application of rhubarb, exhaustive and hungry methods, and treatment groups were treated with extract from Hedysarum polybotrys at 6 g/(kg . d) or Sijunzi decoction at 20 g/(kg . d) for 21 d. Then, general existence,gastrointestinal hormones GAS and MOT levels, and activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase of skeletal muscle were evaluated. Also, confocal laser technology was used to test cellular[Ca2+]i concentrations in skeletal muscle and Western blotting technique was used to test CaM, CaMK II and p-CaMK 11 exp