The expression and significance of heparanase in oral squamous cell carcinoma with different metastatic potentials

To detect the expression of heparanase (HPSE) in oral squamous cell carcinoma (OSCC) with different metastatic potentials and investigate its clinical significance. Transcriptional and translational status of HPSE in OSCC cell lines with different metastatic capacities, primary OSCC samples and thei...

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Published inZhong hua yi xue za zhi Vol. 96; no. 38; p. 3047
Main Authors Wu, X B, Wang, R Y, Gong, Z J, Yu, J, Zhu, S R
Format Journal Article
LanguageChinese
Published China 18.10.2016
Subjects
Carcinoma, Squamous Cell
Cell Movement
Glucuronidase
Humans
Immunohistochemistry
Lymphatic Metastasis
Neoplasm Metastasis
Real-Time Polymerase Chain Reaction
RNA, Messenger
RNA, Small Interfering
Survival Analysis
Up-Regulation
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Summary:To detect the expression of heparanase (HPSE) in oral squamous cell carcinoma (OSCC) with different metastatic potentials and investigate its clinical significance. Transcriptional and translational status of HPSE in OSCC cell lines with different metastatic capacities, primary OSCC samples and their paired metastatic cancer tissues were determined using real-time polymerase chain reaction (PCR) and Western blotting analysis. Immunohistochemistry was used to evaluate HPSE expression in 131 OSCC samples. The correlation between HPSE expression pattern and clinicopathological parameters and clinical outcome in patients with OSCC were analyzed. HPSE level was reduced using HPSE-siRNAs in OSCC cell lines and its impact on cell migration and invasion was measured by scratch assay and matrigel invasion assay. The mRNA and protein levels of HPSE were remarkably up-regulated in OSCC cell lines with highly metastatic capacity ( <0.000 1) and metastatic OSCC tissues ( <0.000 1). The protein levels of HPSE were strongly
ISSN:0376-2491
DOI:10.3760/cma.j.issn.0376-2491.2016.38.003