Bladder reconstruction using autologous bone marrow mesenchyaml stem cells-seeded bladder acellelar matrix graft in a canine model

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 46; no. 1; p. 1
• Main Authors: Yuan, Hai-chao, Tang, Yin, Bai, Yun-jin, Li, Jin-hong, Pu, Chun-xiao, Wang, Kun-jie, Li, Hong, Wei, Qiang, Han, Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2015
• Subjects: Animals; Cystectomy; Cytoskeleton; Dogs; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Muscle, Smooth; Reconstructive Surgical Procedures; Regeneration; Urinary Bladder; Urothelium
• ISSN: 1672-173X

To investigate the feasibility of autologous bone marrow mesenchymal stem cells(BMSCs) seeded on bladder acellular matrix graft (BAMG) for bladder reconstruction in a canine model. This study included 25 mongrel dogs. Five dogs were sacrificed for the preparation of BAMG. Twenty dogs were randomly divided into two groups and received partial cystectomy. In group A, the bladder defect was repaired with unseeded BAMG. In group B, the bladder defect was repaired with autologous BMSCs-seeded BAMG. The bladders were retrieved and studied histologically and immunohistochemically at the time point of 1, 2, 4, 8, 12 weeks after surgery to evaluate tissue regeneration. All dogs survived the procedure. Histopathological examination in group B showed there was urothelium developed at the end of the 2 weeks. By 8 and 12 weeks all bladder wall components were regenerated in the repaired area, which were similar to normal bladder tissue. In group A, urothelium regeneration was observed at the end of the 4 weeks, whereas sm