Effect of Bushen Tongdu Capsule on RANK/RANKL/OPG pathway of collagen induced arthritis rats

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 34; no. 12; p. 1487
• Main Authors: Zhu, Yang-Chun, Lin, Lin, Zhou, Xiao-Li, Li, Rong-Fang, Hou, Li-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2014
• Subjects: Animals; Arthritis, Experimental - metabolism; Drugs, Chinese Herbal - pharmacology; Inflammation; Macrophage Colony-Stimulating Factor - metabolism; Osteoclasts; Osteoprotegerin - metabolism; RANK Ligand - metabolism; Rats; Receptor Activator of Nuclear Factor-kappa B - metabolism; Tripterygium
• ISSN: 1003-5370

To study the effect of Bushen Tongdu Capsule (BTC) on RANK/RANKL/ OPG pathway of collagen induced arthritis (CIA) rats, thereby laying theoretic evidence for treating rheumatic arthritis (RA) by Chinese medicine. RA model was induced by CIA. Totally 42 rats were randomly divided into six groups, i.e., the normal control group, the model group, the low dose BTC (BSL) group, the medium dose BTC (BSM) group, the high dose BTC (BSH) group, and the Tripterygium Glycosides (TG) group, 7 in each group. BTC at the daily dose of 120, 240, and 480 mg/kg was given by gastrogavage to rats in the BSL, BSM, and BSH group respectively from the 13th day of modeling. TG at the daily dose of 24 mg/kg was given by gastrogavage to rats in the TG group. All medication was given once daily, 2 mL each time. Two mL normal saline was administered to rats in the normal control group and the model group. All medication lasted for 18 days. Samples were taken at day 31. The TRAP section of the ankle joint was fixed in 10% formalin for TR