The modification of method of erythrograms in patients under chronic hemodyalisis using hardware-controlled administration of laser low-angle light scattering

• Published in: Klinicheskaia laboratornaia diagnostika no. 4; p. 22
• Main Authors: Borisov, Iu A, Levykina, E N, Mindukshev, I V, Suglobova, E D
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.04.2014
• Subjects: Adult; Erythrocytes - metabolism; Erythrocytes - pathology; Female; Hemolysis; Humans; Kidney Diseases - blood; Kidney Diseases - pathology; Kidney Diseases - therapy; Lasers; Male; Middle Aged; Renal Dialysis; Scattering, Radiation
• ISSN: 0869-2084

The detailed analysis of structural characteristics of erythrocytes can be implemented with method of erythrograms. However its practical application in conditions of medical laboratory is a long and labor-intensive process of little avail for express-diagnostic. To register alterations of morphologic functional characteristics of erythrocytes in patients under chronic dialysis as compared with patients without renal pathology the new technique of low-angle light scattering never applied before for this purpose. Therefore, the purpose of this study is to resolve issue concerning validity of application of this technique for registration of alterations of functional status of erythrocytes in patients of department of chronic dialysis as compared with patients without renal pathology. The experiments concerning the identification of resistance of erythrocytes established significant differences for acid and ammonium models of lysis between patients without renal pathology and patients under chronic dialysis and also in patients in the course of dialysis session. In case of ammonium lysis, the differences were statistically significant between patients without renal pathology and patients under chronic hemodyalisis. In case of acid model, the differences were statistically significant in patients in course of dialysis session. Therefore, the application of low-angle light scattering technique is valid and informative for evaluation of functional status of erythrocytes in patients with terminal stage of chronic renal disease receiving treatment of regular hemodyalisis. The technique itself is low-cost, simple in application and easily reproduced. Therefore, the technique of low-angle light scattering can be applied both in research studies and clinical practice to identify characteristics of stability of membrane systems.