Development of Hf(4+)-immobilized polydopamine-coated magnetic graphene for highly selective enrichment of phosphopeptides

• Published in: Talanta (Oxford) Vol. 149; pp. 91 - 97
• Main Authors: Lin, Haizhu, Deng, Chunhui
• Format: Journal Article
• Language: English
• Published: Netherlands 2016
• Subjects: Animals; Caseins - chemistry; Graphite - chemistry; Hafnium - chemistry; Humans; Indoles - chemistry; Magnetic Phenomena; Milk - chemistry; Phosphopeptides - chemistry; Polymers - chemistry; Serum - chemistry; Serum Albumin, Bovine - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectroscopy, Fourier Transform Infrared; Polydopamine; IMAC; Phosphopeptides enrichment; MALDI; Hafnium
• ISSN: 1873-3573
• DOI: 10.1016/j.talanta.2015.11.037

In this work, we first designed and synthesized an IMAC material with Hf(4+) immobilized on polydopamine-coated magnetic graphene and applied it to phosphopeptides enrichment successfully. The newly prepared material gathered the advantages of large specific surface area of graphene, good hydrophilicity and biocompatibility of polydopamine and superparamagnetism of Fe3O4. We investigated the performance of Hf(4+)-immobilized polydopamine-coated magnetic graphene (denoted as magG@PDA-Hf(4+)) in phosphopeptides enrichment and the results showed high selectivity and sensitivity of the new material. Finally, we successfully applied magG@PDA-Hf(4+) to phosphopeptides enrichment from non-fat milk digests and human serum, further demonstrating excellent performance of this new material in phosphopeptides enrichment.