Establishment of a mouse model of acute graft-versus-host disease by busulfan combined with cyclophosphamide

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 31; no. 12; p. 1637
• Main Authors: He, Xin, Xu, Xiaojun, Wang, Jing, Yi, Wenfang, Ye, Yongbin, Huang, Yuxian, Guo, Kunyuan
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2015
• Subjects: Animals; Bone Marrow Transplantation; Busulfan - administration & dosage; Busulfan - adverse effects; Cyclophosphamide - administration & dosage; Cyclophosphamide - adverse effects; Disease Models, Animal; Female; Graft vs Host Disease - chemically induced; Graft vs Host Disease - mortality; Graft vs Host Disease - pathology; Humans; Liver - pathology; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Spleen - pathology; Transplantation, Homologous
• ISSN: 1007-8738

To develop a stable mouse model of acute graft-versus-host disease (aGVHD) by preconditioning with busulfan (BS)-cyclophospha mide (CP). Male BALB/c (H-2kd) as recipients were conditioned with 100 mg/kg BS and 200 mg/kg CP followed by being transplanted with bone marrow cells (2×10(7)) or bone marrow cells (2×10(7))-spleen cells (2×10(7)) from female C57BL/6(H-2Kb) as donors. The general data of the transplanted mice were recorded and the manifestations of aGVHD were evaluated. Histopathological changes were observed using HE staining and chimerism in spleen and bone marrow cells was studied using flow cytometry. Mice transplanted with bone marrow cells-spleen cells developed classic aGVHD manifestations starting from the 7th day after transplantation with full donor chimerism. GVHD histopathological changes were seen in liver, spleen, intestine, skin and lung of these mice. The median survival time of these aGVHD mice was 10 days, and they all died within 30 days. Mice transplanted with only bone marrow cell