miRNA profile of the human dental pulp cells during odontoblast differentiation induced by BMP-2

To screen and verify the differentially expressed microRNAs (miRNAs) during the differentiation of human dental pulp cells (hDPCs) to odontoblasts induced by BMP-2. The isolated hDPCs were cultured in vitro and induced by BMP-2. The levels of ALP, DMP-1 and DSPP were quantified by quantitative real-...

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Published inShanghai kou qiang yi xue Vol. 26; no. 5; p. 476
Main Authors Bao, Li-Rong, Zhao, Wen-Qing, Lin, Tian, Lu, Yan-Ling, Wu, Yu
Format Journal Article
LanguageChinese
Published China 01.10.2017
Subjects
Bone Morphogenetic Protein 2 - physiology
Cell Differentiation
Cells, Cultured
Dental Pulp - metabolism
Humans
MicroRNAs - metabolism
Odontoblasts
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Summary:To screen and verify the differentially expressed microRNAs (miRNAs) during the differentiation of human dental pulp cells (hDPCs) to odontoblasts induced by BMP-2. The isolated hDPCs were cultured in vitro and induced by BMP-2. The levels of ALP, DMP-1 and DSPP were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). The potential characteristics of hDPCs were investigated by miRNA microarray and highly expressed miRNAs were selected with bio-information software for predicting target genes and their biological functions. Then the results were validated using qRT-PCR analysis for the selected miRNAs. Statistical analysis was performed using SPSS 18.0 software package. The expression of ALP, DSPP, and DMP-1 showed significantly higher levels in BMP-2 induced groups compared to the control group(P<0.05). A total of 36 miRNAs were changed (i.e. 20 miRNAs were up-regulated and 16 were down-regulated). The results of qRT-PCR were consistent with the microarray results. GO categories revealed
ISSN:1006-7248