Effect of Trigonella foenum-graecum 4-hydroxyisoleucine on high-glucose induced insulin resistance in 3T3-L1 adipocytes of mice

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 33; no. 10; p. 1394
• Main Authors: Yu, Hai, Wu, Meng, Lu, Fu-Rong, Xie, Jing, Zheng, Na, Qin, You, Gao, Feng, Du, Wen, Jian, Liu-Meng
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2013
• Subjects: 3T3-L1 Cells; Adipocytes - drug effects; Adipocytes - metabolism; Animals; Glucose - adverse effects; Glucose - metabolism; Insulin - metabolism; Insulin Resistance; Isoleucine - analogs & derivatives; Isoleucine - pharmacology; Male; Mice; Trigonella - chemistry; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1003-5370

To investigate the effect of 4-hydroxyisoleucine (4-HIL), an active component of Trigonella Foenum-graecum L. on high glucose induced insulin resistance (IR) in 3T3-L1 adipocytes, and to explore underlying molecular mechanisms. 3T3-L1 adipocytes were treated with 25 mmol/L glucose and 0.6 nmol/L insulin to induce IR. They were intervened by different concentrations of 4-HIL (at 5, 10, and 20 micromol/L). [3H]-Deoxy-D-glucose up-taking method was used to detect the glucose uptake. The mRNA expression of cellular tumor necrosis factor-alpha (TNF-alpha) was detected by polymerase chain reaction (PCR). The content of TNF-alpha in the culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA). Palmitic acid (PA) acted as the control. After intervened by 25 mmol/L glucose and 0.6 nmol/L insulin for 18 h, the insulin-stimulated glucose transportation in 3T3-L1 adipocytes was inhibited by 63%. The mRNA expression of cellular TNF-alpha in adipocytes significantly increased, when compared with that i