The protective effect of pigment epithelial-derived factor modified human umbilical cord mesenchymal stem cells on rats with diabetic retinopathy

• Published in: Chung-hua yen k'o tsa chih Vol. 53; no. 7; p. 540
• Main Authors: Zhang, W, Duan, H T, Chen, S, Wang, Y X, Kong, J H, Dong, M, Bi, X, Song, J
• Format: Journal Article
• Language: Chinese
• Published: China 11.07.2017
• Subjects: Diabetic retinopathy; Diabetes mellitus, experimental; Vascular endothelial growth factor A; Nerve growth factors; Intravitreal injections; Serpins; Eye proteins; Umbilical cord; Mesenchymal stem cells
• ISSN: 0412-4081
• DOI: 10.3760/cma.j.issn.0412-4081.2017.07.013

To investigate the effect of pigment epithelial-derived factor (PEDF) gene-modified human umbilical cord mesenchymal stem cells (MSC) on rats with diabetic retinopathy (DR). Experimental study. Human umbilical cord MSC were transfected by lentivirus packaging PEDF-MSC-green fluorescent protein (GFP) and GFP-MSC plasmid vectors, and the expression of PEDF and vascular endothelial growth factor (VEGF) was measured in the cell culture medium. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: normal control group (group A), DR control group (group B), phosphate-buffered saline (PBS) treated group (group C), GFP-MSC treated group (group D) and PEDF-MSC-GFP treated group (group E), with 10 rats in each group. Streptozotocin was intraperitoneally injected to make early DR models. After four-month intervention, groups D and E were given intravitreal injection of GFP-MSC and PEDF-MSC-GFP; group C was given intravitreal injection of phosphate-buffered saline; groups A and B did not receive sp