Antitumor efficacy of the recombinant Newcastle disease virus rNDV-IL15 on melanoma models

• Published in: Yao hsüeh hsüeh pao Vol. 49; no. 3; p. 310
• Main Authors: Niu, Ze-Shan, Bai, Fu-Liang, Sun, Tian, Tian, Hui, Yin, Jie-Chao, Cao, Hong-Wei, Yu, Dan, Tian, Gui-You, Wu, Yun-Zhou, Li, De-Shan, Ren, Gui-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2014
• Subjects: Animals; Body Weight; Cell Line, Tumor; Cell Proliferation; Chick Embryo; Cytotoxicity, Immunologic; Female; Genetic Therapy; Interleukin-15 - genetics; Interleukin-15 - metabolism; Melanoma, Experimental - pathology; Melanoma, Experimental - therapy; Mice; Neoplasm Transplantation; Newcastle disease virus - genetics; Plasmids; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Transfection; Tumor Burden
• ISSN: 0513-4870

In order to enhance the antitumor efficacy of recombinant Newcastle disease virus, rNDV-IL15 was rescued in this study. Recombinant plasmid prNDV-IL15 was constructed, and BHK21 cells were transfected with the recombinant plasmid. Finally, the recombinant Newcastle disease virus rNDV-IL15 was successfully rescued. The growth curves of these two recombinant viruses were determined. Murine melanoma B16F10 cells were infected with rNDV-IL15 at MOI of 0.1, and the expression level of IL15 in the supernatant was detected by ELISA. The antitumor efficacy of rNDV-IL15 and rNDV was compared in vitro and in vivo. Results showed that prNDV-IL15 was constructed and recombinant virus rNDV-IL15 was successfully rescued. The growth curve of rNDV-IL15 showed that the growth of rNDV-IL15 had not been changed after insertion of IL15 gene. Results showed that there was high level of IL15 expression in the supernatant of rNDV-IL5-infected B16F10 cells (1 044.3 +/- 27.7 ng x mL(-1)). rNDV-IL15 and rNDV significantly inhibited th