Effect of signal-selective parathyroid hormone analogue peptide on expressions of Wnt signaling factors

• Published in: Zhongguo xiu fu chong jian wai ke za zhi Vol. 26; no. 4; p. 472
• Main Authors: Feng, Ruiqiang, Jiang, Jianming, Li, Junqing, Fu, Zhaozong, Lin, Zhen, Yuan, Liang, Yang, Dehong
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2012
• Subjects: Animals; beta Catenin - genetics; beta Catenin - metabolism; Cells, Cultured; Female; Male; Mice; Mice, Inbred C57BL; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteocalcin - genetics; Osteocalcin - metabolism; Osteogenesis - drug effects; Parathyroid Hormone - analogs & derivatives; Parathyroid Hormone - pharmacology; Parathyroid Hormone-Related Protein - pharmacology; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal Transduction - physiology; Skull - cytology; Wnt Proteins - genetics; Wnt Proteins - metabolism
• ISSN: 1002-1892

To study the effect of signal-selective parathyroid hormone (PTH) analogue peptide on Wnt signaling factors in osteoblasts isolated from neonatal mouse, and provide theoretical basis for the mechanism of PTH's function in bone metabolism. Osteoblasts were isolated from calvaria of 2-3-day-old C57BL neonatal mouse and identified by alkaline phosphatase (ALP) staining, and Alizarin red staining. The cells at passage 1 were divided into 4 groups: control group, PTH (1-34) group, G1R19 (1-34) group, and G1R19 (1-28) group. Then the medium was changed to a-MEM supplemented with 1% FBS. After 12 hours, trifluoroacetic acid or three peptides [(10 nmol/L PTH (1-34), 10 nmol/L G1R19 (1-34), and 100 nmol/L G1R19 (1-28)] were added into the culture medium. After 4 hours, the cells were washed gently with cold PBS 3 times before total RNA was isolated. The expressions of Wnt related genes were measured by quantitative real-time PCR. Most of the cells were polygonal and triangular; the cells were positive for ALP staining