Observation of neuropeptides in bladder after spinal cord injury in rats

• Published in: Zhong hua yi xue za zhi Vol. 92; no. 15; p. 1058
• Main Authors: LI, Rui-zhi, ZHOU, Mou-wang, ZENG, Fan-shuo
• Format: Journal Article
• Language: Chinese
• Published: China 17.04.2012
• Subjects: Animals; Male; Neuropeptide Y - metabolism; Rats; Rats, Sprague-Dawley; Spinal Cord Injuries - metabolism; Substance P - metabolism; Urinary Bladder - metabolism; Urinary Bladder, Neurogenic - metabolism; Vasoactive Intestinal Peptide - metabolism
• ISSN: 0376-2491
• DOI: 10.3760/cma.j.issn.0376-2491.2012.15.013

To observe the altered expressions of neuropeptide Y, substance P and vasoactive intestinal peptide in detrusor of SD rats after spinal cord injury and explore the relationship of the above neuropeptides and neurogenic bladder after spine cord injury. Twenty male clean-grade SD rats, aged 6 weeks, were selected and randomized into spinal cord injury group (n = 10) and control group (n = 10). Rats in spinal cord injury group were smashed at T10 to cause spinal cord incomplete injury model by the weight drop method while laminectomy alone without smashing was administered in control group. At Week 1 post-operation, all rats were assessed by the maximum bladder capacity, bladder compliance and detrusor pressure for the confirmation of spastic bladder. And all detrusor specimens were marked with argentation and immunohistochemistry for the analyses of nerve fibers, neuropeptide Y, substance P and vasoactive intestinal peptide. The results were evaluated with semiquantitative method to observe the contents of nerv