Effects of fluoride on expression of survivin during rats' dental germ development

This study was to investigate the expression of survivin in dental germ development of SD rats with fluorosis, and explore the effects of fluoride on survivin expression and the pathogenic mechanism of dental fluorosis. Forty-five SD rats (pregnant for 10 days) were randomly divided into control gro...

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Published inShanghai kou qiang yi xue Vol. 26; no. 3; p. 254
Main Authors Zhong, Cui-Cui, Tian, Jian-Gang, Huang, Rui-Zhe
Format Journal Article
LanguageChinese
Published China 01.06.2017
Subjects
Animals
Cariostatic Agents - pharmacology
Female
Fluorides - pharmacology
Fluorosis, Dental - etiology
Odontogenesis
Pregnancy
Rats
Rats, Sprague-Dawley
Survivin - metabolism
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Summary:This study was to investigate the expression of survivin in dental germ development of SD rats with fluorosis, and explore the effects of fluoride on survivin expression and the pathogenic mechanism of dental fluorosis. Forty-five SD rats (pregnant for 10 days) were randomly divided into control group, experimental group 1 and group 2. Drinking water with fluoride concentration of 0, 50, 150 mg/L was provided for rats accordingly. The samples were collected at E18.5th day, E20.5th day, P1.5th day, P3.5th day and P5.5th day, then the samples of P1.5th day were selected for H-E staining. SABC method was used for immunohistochemical analysis of samples in each group. The images were acquired by Motic Med 6.0 digital medical image analysis system, and the data were analyzed for ANOVA with SPSS 13.0 software package. Survivin expression fluctuated and exhibited 'M' shape (rose first and fell later) in each group.There was no significant difference at E18.5th day (F=1.050, P>0.05) and E20.5th day (F=2.232, P>0.05)
ISSN:1006-7248