Effect of thyroxine on the expression of HIF-1α after aneurysmal subarachnoid hemorrhage in rat brain and its mechanism

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 36; no. 6; p. 648
• Main Authors: Ran, Hui, Yin, Hao, Liu, Chuang-Xi, Han, Guo-Qiang, Gao, Fang-You, Shen, Hong-Bin, Fu, Hang, Xu, Xiao-Zhong, Li, Tao, Ma, Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2020
• Subjects: Animals; Apoptosis; Brain - metabolism; Male; Phosphatidylinositol 3-Kinases - metabolism; Rats; Rats, Sprague-Dawley; Subarachnoid Hemorrhage - drug therapy; Thyroxine - pharmacology; hypoxia- inducible factor-1α; thyroxine; rat; aneurysmal subarachnoid hemorrhage; apoptosi; PI3K/Akt
• ISSN: 1000-6834
• DOI: 10.12047/j.cjap.5985.2020.135

To investigate the effect of thyroxine (T4) on the expression of hypoxia inducible factor-1α (HIF-1α) in rat brain after aneurysmal subarachnoid hemorrhage (SAH) and its mechanism. Seventy-two adult male SD rats were randomly divided into the following 4 groups: subarachnoid hemorrhage model group(SAH), subarachnoid hemorrhage model and T4 group (SAH with T4), subarachnoid hemorrhage model with normal saline group (SAH with vehicle), and sham-operation group, 18 rats in each group. The model of subarachnoid hemorrhage group was established by internal carotid artery puncture. CT plain scan was performed after the modeling immediately, T4 was administrated by intraabdominal injection of 3 μg/100 g every 24 hours for 3 days. SAH with T4 group was treated with thyroxine. SAH with vehicle group was treated with equal volume vehicle, all of them were killed 72 hours after modeling. The brain water content was determined to evaluate the brain edema, the apoptosis of cerebral cortex cells was detected by TUNEL metho