Study on MSO/GO-based determination method for trace amount of aqueous Hg2

To establish a highly sensitive fluorometric nanobiosensor for determination of aqueous mercury ions (Hg(2+)) using optimized mercury-specific oligonucleotide (MSO) probes and graphene oxide (GO). The nanobiosensor was assembled by attaching the self-designed MSO(1) (5' end labeled with fluorop...

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Bibliographic Details
Published inZhonghua laodong weisheng zhiyebing zazhi Vol. 31; no. 10; p. 783
Main Authors Sun, Tao, He, Jue, Xiang, Xu-wu, Hong, Xiao-lan, Yao, Xiao-yan, Zhang, Lin-zhi, Wang, Yi-yun, Xie, Yan-ne, Wu, Wen-he, Lu, Jian-xin
Format Journal Article
LanguageChinese
Published China 01.10.2013
Subjects
Biosensing Techniques
Fluorometry
Graphite
Mercury - analysis
Nanotechnology
Oligonucleotide Probes
Water
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Summary:To establish a highly sensitive fluorometric nanobiosensor for determination of aqueous mercury ions (Hg(2+)) using optimized mercury-specific oligonucleotide (MSO) probes and graphene oxide (GO). The nanobiosensor was assembled by attaching the self-designed MSO(1) (5' end labeled with fluorophore carboxyfluorescein (FAM), denoted as FAM-MSO(1)) and MSO(2) to the surface of GO through strong non-covalent bonding forces. Upon the addition of Hg(2+), the formation of the T-Hg(2+)-T configuration desorbed the FAM-MSO(1) and MSO(2) from the surface of GO, resulting in a restoration of the fluorescence of FAM-MSO(1). Using the specific mispairing of T-Hg(2+)-T and the changes in fluorescent signals in solutions, quantitative analysis of Hg(2+) could be performed. The average thickness of the prepared GO sheets was only 1.4 nm. For the Hg(2+) nanobiosensor, the optimum concentrations of FAM-MSO(1) and MSO(2) were both 1 µmol/L, the optimum volume of 0.5 g/L GO was 5 µL, and the limit of detection was 10 pmol/L; it
ISSN:1001-9391