Effect of miR-125b on T Cell Activation in Aplastic Anemia by Targetting B7-H4

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 31; no. 6; p. 1797
• Main Authors: Liu, Xiao, Wang, Xue-Xia, Sun, Hong-Kun, Gao, Na, Liu, Zeng-Yan, Liu, Xiao-Dan
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2023
• Subjects: Anemia, Aplastic - genetics; CD40 Ligand - metabolism; Humans; Interleukin-10; Leukocytes, Mononuclear - metabolism; Luciferases; Lymphocyte Activation; MicroRNAs - genetics; RNA, Messenger - metabolism; T-Lymphocytes - metabolism; aplastic anemia; miR-125b; T cell activation; B7-H4
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2023.06.030

To investigate the effect of miR-125b on T cell activation in patients with aplastic anemia (AA) and its molecular mechanism. A total of 30 AA patients were enrolled in department of hematology, Binzhou Medical University Hospital from January 2018 to October 2021, as well as 15 healthy individuals as healthy control (HC) group. Peripheral blood mononuclear cells (PBMCs) were isolated, in which the levels of and mRNA were detected by RT-qPCR. Immunomagnetic beads were used to separate naive T cells and non-naive T cells from AA patients and healthy people to detect the levels of and mRNA. Lentivirus LV-NC inhibitor and LV-miR-125b inhibitor were transfected into cells, and T cell activation was detected by flow cytometry. The dual-luciferase reporter gene assay was used to detect the targetting relationship between miR-125b and B7-H4. RT-qPCR and Western blot were used to detect the levels of mRNA and B7-H4 protein. Compared with HC group, the expression of miR-125b was up-regulated but mRNA was down-regulate