Antiseptic effect of compound lysostaphin disinfectant and its preventive effect on infection of artificial dermis after graft on full-thickness skin defect wound in rats

• Published in: Zhonghua shao shang za zhi Vol. 34; no. 4; p. 225
• Main Authors: Jin, J, Zhou, H, Cui, Z C, Wang, L, Luo, P F, Ji, S Z, Hu, X Y, Ma, B, Wang, G Y, Zhu, S H, Xia, Z F
• Format: Journal Article
• Language: Chinese
• Published: China 20.04.2018
• Subjects: Wound infection; Compound lysostaphin disinfectant; Minimum bactericidal concentration; Muramidase; Minimum inhibitory concentration; Skin, artificial
• ISSN: 1009-2587
• DOI: 10.3760/cma.j.issn.1009-2587.2018.04.007

To study the antiseptic effect of compound lysostaphin disinfectant and its preventive effect on infection of artificial dermis after graft on full-thickness skin defect wound in rats. (1) Each one standard strain of and were selected. Each 20 clinical strains of and were collected from those isolated from wound exudates of burn patients hospitalized in our wards from January 2014 to December 2016 according to the random number table. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of compound lysostaphin disinfectant to above-mentioned strains were detected. The experiment was repeated 3 times. Compared with the corresponding standard strain, the clinical strain with higher MIC and/or MBC was considered as having decreased sensitivity to the disinfectant. The percentage of strains of each of the three kinds of bacteria with decreased sensitivity was calculated. (2) Artificial dermis pieces were soaked in compound lysostaphin disinfectant for 5 min, 1 h, 2 h, and 4 h, r