Establishment and preliminary application of dengue virus envelope domain III IgG antibody capture enzyme-linked immuno-absorbent assay

• Published in: Zhōnghuá yùfáng-yīxué zázhì Vol. 47; no. 4; p. 363
• Main Authors: Hu, Dong-mei, Cai, Jian-piao, Wang, Da-hu, DI, Biao, Qiu, Li-wen, Wang, Ya-di, Chen, Yue, Ding, Xi-xia, Che, Xiao-yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2013
• Subjects: Antibodies, Viral - blood; Dengue - diagnosis; Dengue - immunology; Dengue - virology; Dengue Virus - immunology; Enzyme-Linked Immunosorbent Assay - methods; Humans; Immunoglobulin G - blood; Protein Structure, Tertiary; Sensitivity and Specificity; Seroepidemiologic Studies; Viral Envelope Proteins - immunology
• ISSN: 0253-9624

To establish a highly sensitive and specific assay to detect dengue virus (DENV) envelope protein domain III (EDIII) IgG antibody, and to explore its value in the diagnosis and seroepidemiological survey of dengue. The DENV EDIII IgG antibody capture ELISA was developed using the recombinant full-length DENV EDIII, which was prepared by Pichia yeast expression system as the capture antigen. The serum samples were collected from the same group of 35 DENV-1 patients of primary infection during disease period in 2006 and their follow-up phase in 2010; and the sensitivity of the assay was compared to that of the commercial Panbio DENV IgG ELISA. The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from disease period and follow-up phase was 87% (20/23) and 94% (33/35), respectively; whereas the sensitivity of Panbio DENV IgG ELISA was 71% (25/35) and 0, respectively. The sensitivity of DENV EDIII IgG ELISA in detecting the serum samples from both periods was similar, without statistical signific