Significance of IKZF1 gene copy number abnormalities in BCR/ABL-negative B-lineage acute lymphoblastic leukemia in children

• Published in: Zhongguo dang dai er ke za zhi Vol. 17; no. 11; p. 1154
• Main Authors: Zou, Yao, Liu, Xiao-Ming, Zhang, Li, Chen, Yu-Mei, Guo, Ye, Chen, Xiao-Juan, Yang, Wen-Yu, Wang, Shu-Chun, Ruan, Min, Liu, Tian-Feng, Zhang, Jia-Yuan, Liu, Fang, Qi, Ben-Quan, Zhu, Xiao-Fan
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2015
• Subjects: Adolescent; Child; Child, Preschool; Female; Fusion Proteins, bcr-abl - analysis; Gene Dosage; Humans; Ikaros Transcription Factor - genetics; Infant; Male; Multiplex Polymerase Chain Reaction; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - mortality; Prognosis
• ISSN: 1008-8830
• DOI: 10.7499/j.issn.1008-8830.2015.11.002

To identify IKZF1 gene copy number abnormalities in BCR/ABL-negative B-lineage acute lymphoblastic leukemia (B-ALL) in children, and to investigate the association between such abnormalities and prognosis. Multiplex ligation-dependent probe amplification (MLPA) was applied to detect IKZF1 gene copy number abnormalities in 180 children diagnosed with BCR/ABL-negative B-ALL. These children were classified into IKZF1 deletion group and IKZF1 normal group according to the presence or absence of IKZF1 gene deletion. The association between IKZF1 copy number abnormalities and prognosis of children with BCR/ABL-negative B-ALL was analyzed retrospectively. Among 180 children, 27 (15.0%) had IKZF1 deletion; among the 27 children, 4 had complete deletions of 8 exons of IKZF1 gene, 17 had deletion of exon 1, 3 had deletions of exons 4-7, and 3 children had deletions of exons 2-7. Compared with those in the IKZF1 normal group, children in the IKZF1 deletion group had higher white blood cell (WBC) count and percentage of