Dynamic changes in professional and non-professional antigen presenting cells in the spleen from mice infected with Schistosoma japonicum

• Published in: Zhongguo jishengchongxue yu jishengchongping zazhi Vol. 31; no. 4; p. 298
• Main Authors: Ma, Yi-Lei, Cong, Li, Yin, Lan, Chen, Xiao-Ping
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2013
• Subjects: Animals; Antigen-Presenting Cells - cytology; Basophils - immunology; Dendritic Cells; Eosinophils - immunology; Female; Flow Cytometry; Macrophages - immunology; Mast Cells - immunology; Mice; Mice, Inbred C57BL; Schistosoma japonicum; Schistosomiasis japonica - immunology; Schistosomiasis japonica - parasitology; Spleen - cytology; Spleen - immunology
• ISSN: 1000-7423

OBJECTIVE; To observe the changes in various dendritic cell (DC) subsets, macrophages, basophils, eosinophils and mast cells in mouse spleen before and after Schistosoma japonicum infection induced Th2 response. C57BL6 mice were infected with 20 S. japonicum cercariae via abdominal skin. Before infection and at 2, 4, and 6 weeks post-infection, the mice were sacrificed and spleen was removed. The frequencies of non-T, non-B basophils (NTNB), eosinophils, mast cells, subsets of DC and macrophages in the spleen were measured by flow cytometry. At 4 weeks after infection, when Th2 cells started to occur, the proportion of CD11c+CD8+DC and CD11c+CD4+DC in B220CD11c+DC increased from 7.4% and 7.9% before infection to 17.1% and 12.0%, respectively. During the infection, CD11c+CD4-CD8-DC, the majority of B220CD11c+DC, remained on a nearly constant level (70%); the percentage of B220+ CD11c+ DC in NTNB decreased. The macrophages were subdivided into two subsets: F4/80+CD11b(int) and F4/80+CD11b(high). The percentage