Real-time measurement of noradrenaline release in central nervous system

In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion, the dynamics of catecholamine release is studied in single cell, brain slice or in vivo. Noradrenaline is an important neurotransmitter and modulator in the central nervous system...

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Published inSheng li hsüeh pao Vol. 59; no. 6; p. 865
Main Authors Huang, Wei, Huang, Hong-Ping, Mu, Yu, Zhang, Lei, Jin, Mu, Lv, Jing, Gu, Jing-Li, Xiu, Yun, Zhang, Bo, Guo, Ning, Liu, Tao, Sun, Lei, Song, Mei-Ying, Zhang, Claire Xi, Ruan, Huai-Zhen, Zhou, Zhuan
Format Journal Article
LanguageChinese
Published China 25.12.2007
Subjects
Animals
Central Nervous System - physiology
Ion Channels - physiology
Norepinephrine - secretion
Patch-Clamp Techniques
Rats
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Summary:In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion, the dynamics of catecholamine release is studied in single cell, brain slice or in vivo. Noradrenaline is an important neurotransmitter and modulator in the central nervous system (CNS) and the peripheral nervous system (PNS). In the present paper, we first compared three real-time methods used to measure noradrenaline secretion in single cells (membrane capacitance, amperometry and confocal fluorescence microscopy imaging). Compared to the electrophysiological method and fluorescence microscopy, the basic usage of the carbon fiber electrode (CFE) in neuroscience research was presented as an example. Then, we presented a primary description of ion channels, including voltage-gated Na(+), K(+) and Ca(2+) channels in locus coeruleus (LC) neurons in rat brain slices. Finally, we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons, indicating Ca(2+)-de
ISSN:0371-0874