Simultaneous inhibition of XIAP and survivin expression on EMT and invasion of human pancreatic cancer cells

• Published in: Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 37; no. 9; p. 883
• Main Authors: Zai, Hongyan, Yi, Xiaoping, Li, Yixiong, Jiang, Chun, Lu, Xinsheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2012
• Subjects: Cadherins - metabolism; Cell Line, Tumor; Cell Movement; Epithelial-Mesenchymal Transition - genetics; Humans; Inhibitor of Apoptosis Proteins - genetics; Inhibitor of Apoptosis Proteins - metabolism; Neoplasm Invasiveness; Pancreatic Neoplasms - pathology; Proto-Oncogene Proteins c-akt - metabolism; PTEN Phosphohydrolase - metabolism; Signal Transduction; Snail Family Transcription Factors; Transcription Factors - metabolism; X-Linked Inhibitor of Apoptosis Protein - genetics; X-Linked Inhibitor of Apoptosis Protein - metabolism
• ISSN: 1672-7347
• DOI: 10.3969/j.issn.1672-7347.2012.09.004

To investigate the simultaneous inhibition of X-linked inhibitor of apoptosis protein (XIAP) and survivin expression on epithelial-mesenchymal transition (EMT) and invasiion of pancreatic cancer cells Panc-1, and its mechanism. On the established human pancreatic cancer cells Panc-1-XS, the expression of XIAP and survivin was inhibited simultaneously. Cell invasion and migration were detected by Transwell chamber experiments and scratch test, and the expression of epithelial marker E-cadherin, mesenchymal markers Slug, phosphatase and tensin homolog deleted on chromosome ten (PTEN) and P-Akt protein was determined by Western blot. Cell invasion and migration of Panc-1-XS cells decreased significantly, accompanied by significantly upregulated protein expression of E-cadherin, and significantly declined protein expression of the Slug, indicating increased mesenchymal-epithelial conversion (MET); and increased protein expression of PTEN, and declined protein expression of P-Akt. Simultaneously inhibiting the exp