Proteomic analysis of U937 cells expressing Mycobacterium tuberculosis heat shock protein 16.3

To compare the protein expression differences between U937 macrophages expressing M. tuberculosis (MTB) Hsp16.3 protein and U937 macrophages expressing green fluorescent protein (GFP), and therefore to explore the protein expressions related to latent TB infection(LTBI). U937 macrophages were infect...

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Bibliographic Details
Published inZhonghua jiehe he huxi zazhi Vol. 38; no. 1; p. 34
Main Authors Li, Taiming, Yang, Xingyuan, Meng, Qinglin, Liu, Xiaomei, Zhang, Xia, Zhang, Chun, Zhang, Zongde
Format Journal Article
LanguageChinese
Published China 01.01.2015
Subjects
Bacterial Proteins - metabolism
Heat-Shock Proteins - metabolism
Humans
Macrophages
Mycobacterium tuberculosis - metabolism
Proteome
Proteomics
Tuberculosis
U937 Cells
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Summary:To compare the protein expression differences between U937 macrophages expressing M. tuberculosis (MTB) Hsp16.3 protein and U937 macrophages expressing green fluorescent protein (GFP), and therefore to explore the protein expressions related to latent TB infection(LTBI). U937 macrophages were infected with an integrase-deficient Lentivirus vector to transiently express MTB Hsp16.3, and green fluorescent protein (GFP) as a control. 2-DE was used to compare the differentially expressed proteins in the infected U937 cells. Then 5 significantly different expressed protein spots were identified by using mass spectrometry. The data of 6 protein spots in gel obtained from peptide mass fingerprinting were retrieved in protein database. They were identified as heat shock protein 70, actin, elongation factor I, peptidyl-prolyl cis-trans isomerase, ubiquitin-conjugation enzyme E2, and milk acyl glutathione lyase. The results showed that MTB specific protein intrusion resulted in changes of macrophage proteome expression
ISSN:1001-0939