mRNA transcription of translation elongation factor and primase in different segments of Moniezia expansa

A SYBR green real-time fluorescent quantitative PCR assay was developed for detection of Moniezia expansa mRNA with its beta-tubulin as an internal control. The results showed a good linear relationship (>0.99) between the Ct value and the concentration of positive plasmid for each gene from scol...

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Published inZhongguo jishengchongxue yu jishengchongping zazhi Vol. 30; no. 3; p. 250
Main Authors Wang, Zheng-Rong, Bo, Xin-Wen, Zhao, Wen-Juan, Kang, Li-Chao
Format Journal Article
LanguageChinese
Published China 30.06.2012
Subjects
Animals
Cestoda - genetics
DNA Primase - genetics
Peptide Elongation Factors - genetics
RNA, Messenger - genetics
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Summary:A SYBR green real-time fluorescent quantitative PCR assay was developed for detection of Moniezia expansa mRNA with its beta-tubulin as an internal control. The results showed a good linear relationship (>0.99) between the Ct value and the concentration of positive plasmid for each gene from scolex and various proglottids. Real-time PCR showed that the expression abundance of translation elongation factor and primase was different. In conclusion, the transcription level of translation elongation factor and primase was high in both scolex and immature segment, suggesting that they may play a role in the development of scolex and immature segment.
ISSN:1000-7423