Determining the minimum inhibitory concentration of Tetraclean against Candida albicans

• Published in: Nigerian journal of medicine Vol. 23; no. 3; pp. 201 - 206
• Main Authors: Qyasian, A, Mohammadi, Z, Giardino, L, Palazzi, F, Shalavi, S, Sabbaghi, S, Khoshbin, E
• Format: Journal Article
• Language: English
• Published: Nigeria 01.07.2014
• Subjects: Antifungal Agents - pharmacology; Candida albicans - drug effects; Cetrimonium Compounds - pharmacology; Chlorhexidine - pharmacology; Citric Acid - pharmacology; Doxycycline - pharmacology; Hydrogen Peroxide - pharmacology; Microbial Sensitivity Tests; Polypropylenes - pharmacology; Sodium Hypochlorite - pharmacology
• ISSN: 1115-2613

The purpose of the present study was to determine the minimum inhibitory concentrations of Tetraclean, chlorhexidine, hydrogen peroxide, and sodium hypochlorite against Candida albicans.Amphotericin B was used as positive control and RPMI plus 1 ml Candida suspension was used as negative control. Serial dilution method was used to determine MIC of the irrigants. Findings showed that all positive controls demonstrated complete inhibition of C. albicans at concentration of 0.78 microg mL(-1). On the other hand, all negative controls were positive for fungal growth which confirms the methodology of the study. Findings showed that the MIC of CHX was significantly lesser than other tested irrigants (p < 0.05). The MICs of other groups in an ascending order were as follows: Tetraclean, NaOCl, and H2O2. However, the difference betweenTetraclean and NaOCI was not significant (p > 0.05). It can be concluded that MIC of CHX was significantly lower than other irrigations solutions which confirms its strong antifungal activity.