Production of enzyme preparations on the basis of Penicillum canescens recombinant strains with a high ability for the hydrolysis of plant materials

• Published in: Prikladnaja biohimija i mikrobiologija Vol. 48; no. 1; p. 66
• Main Authors: Volkov, P V, Rozhkova, A M, Pravil'nikov, A G, Andrianov, R M, Dotsenko, G S, Bekkarevich, A O, Koshelev, A V, Okunev, O N, Zorov, I N, Sinitsyn, A P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01.01.2012
• Subjects: Aspergillus niger - enzymology; Aspergillus niger - genetics; beta-Glucosidase - genetics; beta-Glucosidase - metabolism; Cellulase - genetics; Cellulase - metabolism; Cellulose - metabolism; Cellulose 1,4-beta-Cellobiosidase - genetics; Cellulose 1,4-beta-Cellobiosidase - metabolism; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Endo-1,4-beta Xylanases - genetics; Endo-1,4-beta Xylanases - metabolism; Fungal Proteins - genetics; Fungal Proteins - metabolism; Hydrolysis; Kinetics; Metabolic Engineering; Penicillium - enzymology; Penicillium - genetics; Polysaccharides - metabolism; Populus - chemistry; Wood - chemistry
• ISSN: 0555-1099

An enzyme preparation has been produced on the basis of Penicillium canescens strains with the activity of cellibiohydrolase I, II; endo-1,4-beta-gluconase of Penicillium verruculosum; and beta-glucosidase of Aspergillus niger. It was shown that for the most effective hydrolysis of aspen wood pulp the optimal ratio of cellobiohydrolase and endo- 1,4-3-gluconase in enzyme preparations was 8 : 2 (by protein). It was also established that the homologous xylanase secreted by the Penicillium canescens fungus is a required component for the enzyme complex for hydrolysis of the hemicellulose matrix of aspen wood.