Transformations of dinitrosyl iron complexes in an isolated rat heart after introduction of this substance into perfusion medium

The objective of the present research was to study transformations of various physiological NO forms in an isolated rat heart, perfused with the medium, containing dinitrosyl iron complexes with glutathione ligand (DNIC-GH). We showed that such aerobic perfusion resulted in accumulation of mostly di...

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Bibliographic Details
Published inBiofizika Vol. 58; no. 2; p. 281
Main Authors Timoshina, A A, Lakomkin, V L, Drobotova, D Iu, Ruuge, E K, Vanin, A F
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2013
Subjects
Animals
Electron Spin Resonance Spectroscopy
Glutathione - chemistry
Heart - drug effects
Iron - chemistry
Iron - pharmacology
Ischemia
Ligands
Mitochondria, Heart - chemistry
Mitochondria, Heart - drug effects
Myocardium - chemistry
Nitric Oxide - chemistry
Nitrogen Oxides - chemistry
Nitrogen Oxides - pharmacology
Organ Culture Techniques
Perfusion
Quinones - chemistry
Rats
S-Nitrosothiols - chemistry
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Summary:The objective of the present research was to study transformations of various physiological NO forms in an isolated rat heart, perfused with the medium, containing dinitrosyl iron complexes with glutathione ligand (DNIC-GH). We showed that such aerobic perfusion resulted in accumulation of mostly diamagnetic NO physiological forms (S-nitrosothiols) in myocardial tissue. They were transformed into protein-bound mononuclear dinitrosyl iron complexes during subsequent total ischemia. Meantime, DNIC-GH injection on the onset of ischemia resulted in the changes in the state of mitochondrial respiratory state, characterized by the increase in myocardial concentration of flavosemiquinones.
ISSN:0006-3029