Qibaipingfei Capsule down-regulate the levels of calcineurin and nuclear factor of activated T-cells isoform c3 (NFATc3) in chronic obstructive pulmonary disease

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 31; no. 11; p. 1497
• Main Authors: Zhu, Jie, Wang, Baoqin, Li, Zegeng, Lu, Mei, Peng, Qinghe, Yang, Cheng, Tong, Jiabing
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2015
• Subjects: Animals; Calcineurin - analysis; Capsules; Down-Regulation; Drugs, Chinese Herbal - pharmacology; Male; NFATC Transcription Factors - analysis; Pulmonary Disease, Chronic Obstructive - drug therapy; Pulmonary Disease, Chronic Obstructive - metabolism; Pulmonary Disease, Chronic Obstructive - pathology; Rats; Rats, Sprague-Dawley
• ISSN: 1007-8738

To investigate the effect of Qibaipingfei Capsule (QPC) on the expressions of calcineurin (CaN) and nuclear factor of activated T-cells isoform c3 (NFATc3) of rat models with phlegm and blood stasis syndrome of chronic obstructive pulmonary disease (COPD), and to explore the possible mechanism underlying the intervention of QPC in pulmonary vascular remodeling of COPD. Sixty male Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group, a positive group of nifedipine, a high dose group, a middle dose group and a low dose group of QPC. The rat models with phlegm and blood stasis syndrome of COPD were established by compound methods of forced swimming, smoking and hypoxia. Then the pulmonary function and the pathological alterations of pulmonary vessels were observed. Furthermore, the mRNA and protein levels of CaN and NFATc3 in the lung tissues were determined by real-time quantitative PCR and Western blot analysis. Compared with the normal group, the forced expiratory volume at 0.3 se