Diversity study of arbuscular mycorrhizal fungi of Coptis deltoidea

• Published in: Zhong yao cai Vol. 35; no. 5; p. 689
• Main Authors: Huang, Wen-li, Fan, Xin-jian, Yan, Zhu-yun, Ma, Yun-tong, Meng, Xian-li
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2012
• Subjects: Biodiversity; Colony Count, Microbial - methods; Conservation of Natural Resources; Coptis - growth & development; Coptis - microbiology; Ecosystem; Fungi - classification; Fungi - isolation & purification; Fungi - physiology; Mycorrhizae - classification; Mycorrhizae - isolation & purification; Mycorrhizae - physiology; Plant Roots - growth & development; Plant Roots - microbiology; Soil Microbiology; Spores, Fungal - classification; Spores, Fungal - isolation & purification; Spores, Fungal - physiology
• ISSN: 1001-4454

To investigate the resources and distribution of Arbuscular Mycorrhizal Fungi (AMF) in rhizosphere of Coptis deltoidea. The colonization situation of AMF of Coptis deltoidea were detected by acid fuchsin staining and then calculated the colonization rate. AMF fungal spores were obtained by Wet-screening method and then classified and identified by their morphological characteristics and histochemical staining method. Coptis deltoidea and AMF could engender the mycorrhiza. Colonization rates were different in different producing areas and ranged from 23. 3% to 34.4%. Thirty species of AMF belonging in 6 genera were found, including 17 species of the Acaulospora, 7 of the Glomus, 2 of the Gigaspora, 1 of the Entrophospora, 1 of the Paraglomus and 2 of the Archaeospora were isolated from rhizosphere of Coptis deltoidea. Acaulospora and Glomus were the dominant genera and the AMF species of genuine producing regions in Hongya county was the most diversiform among the six areas. Coptis deltoidea is a kind of nutri