Bioavailability in vivo and in vitro transepithelial transport across Caco-2 cell monolayer of pueraria flavonoids bio-adhesive and floating pellets

• Published in: Yao hsüeh hsüeh pao Vol. 51; no. 7; p. 1144
• Main Authors: Li, Ying, Song, Yi-qun, He, Ya-li, Zhu, Chun-yan
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2016
• Subjects: Adhesives - chemistry; Biological Availability; Biological Transport; Caco-2 Cells; Chromatography, High Pressure Liquid; Chromatography, Liquid; Flavonoids - pharmacokinetics; Humans; Hypromellose Derivatives - chemistry; Isoflavones - pharmacokinetics; Pueraria - chemistry; Software
• ISSN: 0513-4870
• DOI: 10.16438/j.0513-4870.2016-0146

To study the bioavailability of pueraria flavonoids bio-adhesive and floating pellets, the absorption of puerarin was studied using Caco-2 cell monolayer by liquid chromatography (HPLC) method, comparing the P(app) of pueraria flavonoids bio-adhesive and floating pellets with different bio-adhesive materials. Drugs were administered at a dose of 100 mg·kg(-1) via ig. The plasma concentration of puerarin was determined by HPLC, the pharmacokinetics were calculated with the WinNonlin 6.0 software. The results showed that the P(app) of bio-adhesive and floating pellets with hydroxypropyl methylcellulose (HPMC)-cabomer was largest, which had a significant difference (P < 0.05). The AUC(0-t) of pueraria flavonoids bio-adhesive and floating pellets was 1.79 times of pueraria flavonoids, the C(max) of pueraria flavonoids bio-adhesive and floating pellets and pueraria flavonoids had a significant difference (P < 0.05). What’s more the MRT had prolonged. In conclusion, pueraria flavonoids bio-adhesive and floating pel