Effect of Interferon Combined with Thalidomid on HEL Cell Apoptosis and JAK2V617F Mutation Gene Expression

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 24; no. 4; p. 998
• Main Authors: Wu, Xiao-Xie, Xue, Lian-Guo, Zhao, Li-Dong, Wang, Ying, Cai, Zhi-Mei
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2016
• Subjects: Apoptosis; Cell Line, Tumor; Gene Expression; Gene Expression Regulation, Neoplastic; Humans; Interferon-alpha; Janus Kinase 2; Mutation; Thalidomide
• ISSN: 1009-2137
• DOI: 10.7534/j.issn.1009-2137.2016.04.007

To investigate the effects of interferon-α2b combined with thalidomid(THD) on the proliferation and apoptosis of HEL cells, and expression of JAK2V617F-mutated gene. The cell survival rates were assayed by CCK-8 after HEL cells were treated by interferon-α2b, thalidomid and thalidomid combined with interferon-α2b for 24, 48 and 72 hours, while the apoptosis and expression of JAK2V617F mutation gene were detected by flow cytometry and fluorescence quantitative PCR respectively after treatmemt for 48 hours. IFN-α2b combined with THD could more obviously inhibit the proliferation of HEL cells than IFN-α2b or THD alone for 24 and 48 and 72 hours, and the differences between groups were statistically significant(P<0.05). In addition, the apoptosis-inducing effect of IFN-α2b combined with THD on HEL cells was more obvious than that of IFN-α2b used alone, the differences was statistically significant(P<0.05). Although IFN-α2b combined with THD can decrease the JAK2V617F mutation gene expression more obviously than I